|
KMID : 0385520130260010034
|
|
Analytical Science & Technology
2013 Volume.26 No. 1 p.34 ~ p.41
|
|
|
Investigation on sample throughput of large scale splitter-less gravitational SPLITT fractionation (GSF)
|
|
Choi Hyo-Jae
Lee Seung-Ho
Kim Woon-Jung
Eum Chul-Hun
|
|
|
Abstract
|
|
|
Split-flow thin cell (SPLITT) fractionation (SF) is a rapid separation technique capable of separating colloidal particles or macromolecules into two or more fractions. SF allows fractionations in a preparative scale as sample is fed continuously. Generally SF uses a thin ribbon-like channel equipped with two flow stream splitters at the inlet and outlet of the channel. Thus there exist two flow inlets and two flow outlets at the top and bottom of the inlet and outlet of the channel, respectively. There are two operating modes in SF, conventional mode and full-feed mode (FFD). Although the resolution in the FFD mode is lower than that in the conventional mode, FFD mode has some merits. The design of the channel and operation are simpler in the FFD mode, as it does not require the feeding of the solvent. Thus there is no flow stream splitter at the channel inlet, and only one pump is needed, unlike the conventional mode, where two pumps are required
for the feedings of the sample and the solvent separately. Also the sample is not diluted in the FFD mode as there is no solvent feeding, which is important for fractionation samples with low colloidal concentrations such as environmental samples. For some of environmental samples, pre-concentration is often required. In this study, a new large-scale splitter-less FFD-SF channel was implemented, where there is no splitter at the outlet as well as at the inlet of the channel. It was possible to build the channel in a much larger dimension than conventional ones, allowing much higher sample throughput (TP). The new channel was tested and optimized with polyurethane (PU) latex beads, and then applied to large-scale separation of Polyacrylate (PA).
|
|
|
KEYWORD
|
|
|
SPLITT, FFD throughput, polyurethane
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|